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KMID : 1234420110390010077
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Korean Journal of Microbiololgy and Biotechnology
2011 Volume.39 No. 1 p.77 ~ p.85
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Antibacterial Activity of Silver-nanoparticles Against Staphylococcus aureus and Escherichia coli
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Kim Soo-hwan
Lee Hyung-seon
Ryu Deok-Seon
Choi Soo-jae
Lee Dong-Seok
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Abstract
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The antibacterial activities of silver nanoparticles (Ag-NPs) were studied with respect to Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli by observing the bacterial cells treated or not with Ag-NPs by field emission scanning electron microscope (FE-SEM) as well as measuring the growth curves, formation of bactericidal reactive oxygen species (ROS), protein leakage, and lactate dehydrogenase activity involved in the respiratory chain. Bacterial cells were treated with Ag-NPs powder, and the growth rates were investigated under varying concentrations of Ag-NPs, incubation times, incubation temperatures, and pHs. As a result, S. aureus and E. coli were shown to be substantially inhibited by Ag-NPs, and the antibacterial activity of Ag-NPs did not fluctuate with temperature or pH. These results suggest that Ag-NPs could be used as an effective antibacterial material.
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